StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

Fruit content of fruit juice and apple juice content of cider using DNA methodology - Dissertation Example

Cite this document
Summary
Fruit content of fruit juice and apple juice content of cider using DNA methodology Introduction: The soft fruit juices contain a lot of bioactive products. These compounds enhance the body functions. The fruit juices contain vitamins and minerals and thus act as a smart addition to the well balanced diet…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER92.8% of users find it useful
Fruit content of fruit juice and apple juice content of cider using DNA methodology
Read Text Preview

Extract of sample "Fruit content of fruit juice and apple juice content of cider using DNA methodology"

Download file to see previous pages

The people want the analytical techniques to be very quick and easy for the identification of the honesty and accuracy of the ingredients. Many modern analytical techniques are used for the analysis of the plant or animal species in the foods. The techniques based on DNA are much common nowadays. The people have lost their hope on chemical analysis as they cannot predict the correct results because of the varying processing methods. DNA is resistant to the food processing method hence this can used for the analysis.

Most of the molecular genetic techniques are based on the PCR, pyrosequencing and CAPS (Cleavable Amplifiable polymorphic Sites). Aim of the project: To determine the fruit content of the fruit juice, using the DNA methodologies. Main work packages: The fruit juices are selected and tested for the fruit content based on the value of the juice and to check the level of fraudulent substitution of other fruits in the juice using the DNA methodologies. The high value fruit juices are accidentally or fraudulently substituted with the other fruits.

This affects the trustworthy of the product in the customers mind. Hence a novel technique that can identify these fraudulent are required for the prevention. (Bauer et al. 2003). Outline of the project: The fruit juices were obtained from the commercial juice producers. The fresh juices were mixed thoroughly by shaking and inverting. (Doyle and Doyle 1987). The fruit juices are then diluted with the ultra pure water. This dilution is done to reduce the percentage of sugar in the juice. The DNA was extracted from the fruit juices using the Standard CTAB DNA extraction method.

(Kress et al. 2005). One ml of the sample was suspended in the 5 ml of the CTAB buffer, 100mmTris HCl, 20mM EDTA, 1.4M NaCl and 40µl of proteinase K solution. It was vigorously shaked ad stored at 60 degree Celsius overnight. (Doyle and Doyle 1987). The clear supernatant was removed and added with equal volume of chloroform and centrifuged and the supernatant was collected. Equal volume of isopropanol was added to precipitate DNA. The pelleted DNA was washed with ethanol and dried and stored in the 1X TE buffer.

The concentration of the DNA was estimated using suitable methods. The DNA obtained was amplified before moving to the PCR. The amplified product was confirmed with the gel electrophoresis. The design of the primer is an important part of PCR. The primers for the psbA-trnH chloroplast are used for the Taberlet PCR. (Delano and Schmidt 2004). The products can be amplified using the Taberlet PCR and used for further studies. The PCR products were then run in the gel electrophoresis. The DNA fragments were separated in the gel electrophoresis and the gel images are captured in the Gel Doc.

The DNA are then separated and extracted from the gel and the restriction enzymes are used for the further analysis. The restriction enzymes are specific for the species. The restriction enzymes that can be used for fruit juice analysis are Acil, Apol, Dbel, Mbol,Mnll, NlaIII, TaqAl etc., (Taberlet et al. 1991). The Full length of the DNA can be found by aligning the contigs of the sample. Consensus sequences are generated using the Bioinformatics tools. These consensus sequences can then be used for the analysis of PCR-RFLP patterns.

The Apol and Dbel are used for the analysis of the six different fruit species such as apple, blueberry, elderberry, grape, pear and pomegranate. If Apol

...Download file to see next pages Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Fruit content of fruit juice and apple juice content of cider using Dissertation”, n.d.)
Retrieved from https://studentshare.org/gender-sexual-studies/1417026-fruit-content-of-fruit-juice-and-apple-juice
(Fruit Content of Fruit Juice and Apple Juice Content of Cider Using Dissertation)
https://studentshare.org/gender-sexual-studies/1417026-fruit-content-of-fruit-juice-and-apple-juice.
“Fruit Content of Fruit Juice and Apple Juice Content of Cider Using Dissertation”, n.d. https://studentshare.org/gender-sexual-studies/1417026-fruit-content-of-fruit-juice-and-apple-juice.
  • Cited: 0 times

CHECK THESE SAMPLES OF Fruit content of fruit juice and apple juice content of cider using DNA methodology

Excellent Fruit Juice Co Industry Analysis

hellip; Its core business would be to produce 100% natural fresh fruit juice and fruit juice, which would be sold to people living in Newcastle, North of England.... There is need for 100% fruit juice and fresh fruits to improve livelihoods of people of Newcastle.... (EFJC) is a public company, which produce fresh fruit juice and fruit juice.... Its core business is to produce both fresh fruit juice and fruit juice from a variety of fresh fruits available in the UK....
15 Pages (3750 words) Essay

Estimation of Ascorbic Acid in Fruit Juices

The fruit juice sample is treated with metaphosphoric acid.... Reagents and samples: 2,6-dichlorophenolindophenol (DCP), 2- metaphosphoric acid, glacial acetic acid, standard ascorbic acid, freshly squeezed orange juice, Tropicana pure premium, and Growers direct organic Accurately weighed 800 mg of DCP is dissolved in 500ml of previously boiled and cooled distilled water in a 1000 ml volumetric flask.... In the paper “Estimation of Ascorbic Acid in fruit Juices,” the author determines the amount of ascorbic acid in orange juices....
5 Pages (1250 words) Research Proposal

Evaluate and revise A Strategy for Scottish Tourism

It examines the immediate internal and external environmental factors that shape the strategy and also arrives at specific recommendations for improving the same.... hellip; Since tourism is recognized as a major contributor to the Scottish economy, government efforts to promote and sustain tourism in the nation is a continuous process and due to changing environment conditions that make for a very competitive tourism industry, it was felt expedient It was evolved primarily based on the views that the overseas target markets showed declining performance, there was considerable increase in short tours and also the expenditure was exceeding budgets as per the targets set....
16 Pages (4000 words) Essay

Value Stream Mapping of Vibration Test Data in a Product Life Cycle

LPD (Lean Product Development) can have significant impact by reducing time and costs.... For this purpose, Value Stream Mapping (VSM) is… By implementing the lean transformation, value is enhanced and non-value is either minimised, or in the case of pure waste, eliminated.... The Applying the lean principles is an important part of the stages where the product development takes place....
33 Pages (8250 words) Thesis

The Business Opportunity of the Fresh Fruit Juice Market

The company is a new player in the provision of fresh fruit juice in Halifax.... hellip; Fresh fruit juice business is hot in Halifax.... Increasing population take more fresh fruit juice because it is considered super healthy and very tasty as compared to flavored juice.... Fortunately, there are no fresh fruit juice vendors around.... The fresh fruit juice market is one of the most promising segments.... The business opportunity was conceived when the owner realized that most people preferred fresh fruit juice to the flavored....
20 Pages (5000 words) Assignment

Fruiti Oasis Juice Bar Business Plan

Fruiti Oasis aspires to become the leading healthy fruit juice producer in Kuwait.... The fruit bar is going to produce high quality and natural fruit juice using three different fruits appropriate for every age group and will be based on the fact that fruits and fruit juices are popular among Kuwaitis.... The paper contains a business plan for Fruiti Oasis juice Bar which fundamental responsibility will be to offer customers the healthiest and most delicious fruit juices that will be the epitome of true refreshment....
17 Pages (4250 words) Assignment

Development Plan for a Fruit Juice Stand

This report “Business Plan for a Fruit Juice Stand” is based on a business plan for my fruit juice and smoothie stand inside the Westfield Stratford shopping centre.... Questions ranged from the respondents' likeness of the fruit juice and smoothies to their daily intake of soft drinks and packed carbonated drinks.... However, there was a sign of hope when the question mentioned of a possibility of a fruit juice and smoothies contained in beautiful glasses, in which the respondents positively answered that they would prefer fruit juice and smoothies if these are readily available in stores inside the mall....
38 Pages (9500 words) Dissertation

The Description of the Company and Product - Fresh Fruit Juice

This paper "The Company of Fresh fruit juice and Its Products" focuses on the BestJuice Limited - the brainchild of Assem, who holds Bachelors Degree in Food Science and Technology with over two-year experience in food production.... nbsp; BestJuice Limited was established to take on growth opportunities in the fresh fruit juice market.... The fresh fruit juice segment is on the growth rate and promises higher returns to investors.... Every segment of fresh fruit juices grew significantly and it is estimated that fresh fruit juice segment will grow at the rate of 4% - 6% annually in the next decade (Jackson, 2008)....
6 Pages (1500 words) Assignment
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Contact Us